1GAQ

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN FERREDOXIN AND FERREDOXIN-NADP+ REDUCTASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.070, 93.410, 135.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.560 - 2.590
R-factor	0.233 *
Rwork	0.232
R-free	0.29700
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.850
High resolution limit [Å]	2.590 *	2.590 *
Rmerge	0.098 *	0.255 *
Total number of observations	319768 *
Number of reflections	23300 *
Completeness [%]	97.8 *	96.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 4000, Tris, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir		100 (mM)
4	1	reservoir	Tris-HCl	50 (mM)