1GAM
GAMMA B CRYSTALLIN TRUNCATED C-TERMINAL DOMAIN
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 36.480, 36.480, 232.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.020 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.070 |
| Total number of observations | 43347 * |
| Number of reflections | 9345 |
| Completeness [%] | 95.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.35 * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | gammaB-C | 0.8 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
