1GAD

COMPARISON OF THE STRUCTURES OF WILD TYPE AND A N313T MUTANT OF ESCHERICHIA COLI GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASES: IMPLICATION FOR NAD BINDING AND COOPERATIVITY

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	79.140, 189.560, 122.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.197
Rwork	0.197
RMSD bond length	0.008
RMSD bond angle	1.620
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	1.800	1.800 *
Rmerge	0.065 *
Number of reflections	66191 *
Completeness [%]	69.0 *	40 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	tris-sodium citrate	1.35 (M)
2	1	reservoir	EDTA	1 (mM)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	azide	0.1 (mM)
5	1	reservoir	NAD	0.3 (mM)
6	1	reservoir	HEPES	100 (mM)