1G9W
STRUCTURAL BASIS OF COLLAGEN STABILIZATION INDUCED BY PROLINE HYDROXYLATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.887, 26.340, 20.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.147 |
| R-free | 0.19400 |
| Starting model (for MR) | PDB1A3J.ENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.042 | 0.093 |
| Number of reflections | 3448 | |
| Completeness [%] | 89.0 | 66.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 293 | Kramer, R.Z., (1998) J.Mol.Biol., 280, 623. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 7.5 (mg/ml) | |
| 2 | 1 | drop | aqueous acetic acid | 5 (%(v/v)) | |
| 3 | 1 | reservoir | acetate | 0.1 (M) |
