1G9U
CRYSTAL STRUCTURE OF YOPM-LEUCINE RICH EFFECTOR PROTEIN FROM YERSINIA PESTIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 109.359, 109.359, 101.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.350 |
| R-factor | 0.17 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MIR |
| Starting model (for MR) | new structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 2.450 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 39896 | |
| <I/σ(I)> | 9 | 2.2 |
| Completeness [%] | 81.0 | 96.4 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | Evdokimov, A.G., (2000) Acta Crystallog., D56, 1676. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2-propanol | 10 (%) | |
| 2 | 1 | reservoir | 450 (mM) | ||
| 3 | 1 | reservoir | sodium-MES | 100 (mM) | |
| 4 | 1 | reservoir | ethylene glycol | 20 (%) | |
| 5 | 1 | drop | protein | 6-8 (mg/ml) |
