1G9O
FIRST PDZ DOMAIN OF THE HUMAN NA+/H+ EXCHANGER REGULATORY FACTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98401 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 51.568, 51.568, 58.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.600 - 1.500 |
| R-factor | 0.181 * |
| Rwork | 0.181 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.039 | 0.152 |
| Total number of observations | 246628 * | |
| Number of reflections | 14916 | |
| <I/σ(I)> | 35 | 10.3 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 7.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 20 * | potassium sodium tartrate, sodium citrate, ammonium sulfate, manganese chloride, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | potassium sodium tartrate | 0.05 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.06 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 5 | 1 | reservoir | 20 (mM) |
