1G93
CRYSTAL STRUCTURE OF THE BOVINE CATALYTIC DOMAIN OF ALPHA-1,3-GALACTOSYLTRANSFERASE IN THE PRESENCE OF UDP-GALACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-10 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9324 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 95.608, 95.608, 110.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | native crystal structure |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.043 | 0.227 |
| Total number of observations | 452447 * | |
| Number of reflections | 18010 | |
| <I/σ(I)> | 9.3 | 2.4 |
| Completeness [%] | 99.5 * | 99.5 * |
| Redundancy | 6.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 1.3 to 1.6 M Sodium Acetate, 10 mM MnCl2, 10 mM Hg-UDP-galactgose, 500 mM NaCl, 100 mM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | UMP | 10 (mM) | |
| 5 | 1 | drop | 500 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 1.3-1.6 (M) | |
| 7 | 1 | reservoir | cacodylate | 50 (mM) |
