1G8Y
CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9059 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.432, 179.156, 116.289 |
| Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.221 * |
| Rwork | 0.221 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.034 * | 0.117 |
| Total number of observations | 377676 * | |
| Number of reflections | 149192 | |
| <I/σ(I)> | 18.9 | 6.9 |
| Completeness [%] | 94.6 | 81.7 |
| Redundancy | 2.53 | 2.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | PEG 5000 monomethyl ether, 2-methyl-2,4-pentanediol, NaCl, sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 38 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | PEG5000 MME | 12 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 3 (%(v/v)) | |
| 6 | 1 | reservoir | 150 (mM) | ||
| 7 | 1 | reservoir | sodium citrate | 100 (mM) |
