1G8W
IMPROVED STRUCTURE OF PHYTOHEMAGGLUTININ-L FROM THE KIDNEY BEAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-08-01 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.479, 121.278, 89.957 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LES (lentil lectin) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | MADNESS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.107 | |
| Total number of observations | 105939 * | |
| Number of reflections | 26546 * | |
| <I/σ(I)> | 11.23 | 3.2 |
| Completeness [%] | 94.4 | 82.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 293 | 5 mg/ml protein; 100 mM TRIS/HCl; 8% (w/v) PEG(6000), pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | protein | 5-7 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 8-12 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2-0.4 (M) |
