1G8Q
CRYSTAL STRUCTURE OF HUMAN CD81 EXTRACELLULAR DOMAIN, A RECEPTOR FOR HEPATITIS C VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.485, 77.172, 38.462 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.187 * |
| Rwork | 0.188 |
| R-free | 0.23800 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.038 | 0.303 |
| Number of reflections | 21557 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 98.0 | 93.1 * |
| Redundancy | 6.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 * | PEG 4000, MES, NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | MES | 0.1 (M) |
