1G8Q

CRYSTAL STRUCTURE OF HUMAN CD81 EXTRACELLULAR DOMAIN, A RECEPTOR FOR HEPATITIS C VIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-19
Detector	MARRESEARCH
Wavelength(s)	0.93
Spacegroup name	P 1 21 1
Unit cell lengths	31.485, 77.172, 38.462
Unit cell angles	90.00, 107.39, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.187 *
Rwork	0.188
R-free	0.23800
Structure solution method	MIR
RMSD bond length	0.006 *
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.038	0.303
Number of reflections	21557
<I/σ(I)>	5.2
Completeness [%]	98.0	93.1 *
Redundancy	6.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293 *	PEG 4000, MES, NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir		0.1 (M)
4	1	reservoir	MES	0.1 (M)