1G8Q
CRYSTAL STRUCTURE OF HUMAN CD81 EXTRACELLULAR DOMAIN, A RECEPTOR FOR HEPATITIS C VIRUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.485, 77.172, 38.462 |
Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.187 * |
Rwork | 0.188 |
R-free | 0.23800 |
Structure solution method | MIR |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.038 | 0.303 |
Number of reflections | 21557 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 98.0 | 93.1 * |
Redundancy | 6.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 * | PEG 4000, MES, NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
3 | 1 | reservoir | 0.1 (M) | ||
4 | 1 | reservoir | MES | 0.1 (M) |