1G8P
CRYSTAL STRUCTURE OF BCHI SUBUNIT OF MAGNESIUM CHELATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.958 |
| Spacegroup name | P 65 |
| Unit cell lengths | 90.259, 90.259, 83.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.550 - 2.100 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.042 | 0.246 |
| Number of reflections | 22179 | |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 97.9 | 100 * |
| Redundancy | 14 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 2000, Tris , MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 16 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) |
