1G8P

CRYSTAL STRUCTURE OF BCHI SUBUNIT OF MAGNESIUM CHELATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-05-17
Detector	MARRESEARCH
Wavelength(s)	0.958
Spacegroup name	P 65
Unit cell lengths	90.259, 90.259, 83.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.550 - 2.100
R-factor	0.214
Rwork	0.214
R-free	0.24700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	0.246
Number of reflections	22179
<I/σ(I)>	25.6
Completeness [%]	97.9	100 *
Redundancy	14	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	PEG 2000, Tris , MgCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG2000	16 (%(w/v))
3	1	reservoir	Tris-HCl	0.1 (M)