1G8A
PYROCOCCUS HORIKOSHII FIBRILLARIN PRE-RRNA PROCESSING PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2000-06-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.376, 41.098, 54.168 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fib |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO (SUITE) |
| Data scaling software | SCALEPACK (SUITE) |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.033 | 0.060 |
| Number of reflections | 37696 | |
| <I/σ(I)> | 40 | |
| Completeness [%] | 94.2 | 71.2 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Cooling | 7.5 | 277 | 50 mM NaCl, 50 mM Tris, 85 mg/ml fibrillarin, pH 7.5, Cooling, temperature 4K |
