1G88

S4AFL3ARG515 MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-03-01
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	140.855, 140.855, 194.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.500 - 3.000
R-factor	0.203
Rwork	0.203
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.126	0.485
Total number of observations	72845 *
Number of reflections	19552
<I/σ(I)>	9.5	5.5
Completeness [%]	97.9	88
Redundancy	3.72	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	298	pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	HEPES	100 (mM)
2	1	2	lithium sulfate	250 (mM)
3	1	2	PEG4000	10 (%(v/v))