1G87
THE CRYSTAL STRUCTURE OF ENDOGLUCANASE 9G FROM CLOSTRIDIUM CELLULOLYTICUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.850, 57.720, 86.270 |
| Unit cell angles | 94.22, 100.87, 99.61 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1js4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.058 * | 0.451 * |
| Number of reflections | 135088 | |
| <I/σ(I)> | 14 | 1.6 |
| Completeness [%] | 97.0 | 95.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 * | 17 * | PEG 4000, Tris, Mg Acetate, Isopropanol, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.4 |
| 3 | 1 | reservoir | magnesium acetate | 100 (mM) | |
| 4 | 1 | reservoir | isopropanol | 5 (%) | |
| 5 | 1 | reservoir | glycerol | 15 (%) | |
| 6 | 1 | drop | protein | 6.25 (mg/ml) |
