1G82

STRUCTURE OF FIBROBLAST GROWTH FACTOR 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12-22
Detector	MARRESEARCH
Wavelength(s)	1.07
Spacegroup name	I 41
Unit cell lengths	151.947, 151.947, 117.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.2103
Rwork	0.208
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2afg
RMSD bond length	0.034
RMSD bond angle	2.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.057	0.242
Number of reflections	40985
<I/σ(I)>	8.5	3.3
Completeness [%]	99.9	99.9 *
Redundancy	6.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	292	Ammonium sulfate, MES, TRIS, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.1 (mg/ml)
2	1	drop	ammonium sulfate	2.0 (M)
3	1	drop	MES/Tris	0.1 (M)