1G81
CHORISMATE LYASE WITH BOUND PRODUCT, ORTHORHOMBIC CRYSTAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.962, 62.983, 71.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.710 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.120 |
| Total number of observations | 63214 * | |
| Number of reflections | 15812 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 95.0 | 83 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 4 * | Stover, C., (2000) J. Struct. Biol., 129, 96. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 26 (%(v/v)) | |
| 2 | 1 | reservoir | HEPES | 80 (mM) | |
| 3 | 1 | reservoir | isopropanol | 5 (%(v/v)) | |
| 4 | 1 | reservoir | protein | 5 (mM) |
