1G7S
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-04-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.430, 54.523, 91.860 |
| Unit cell angles | 104.54, 100.18, 99.04 |
Refinement procedure
| Resolution | 22.000 - 2.000 |
| Rwork | 0.220 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.150 |
| Number of reflections | 53169 | |
| <I/σ(I)> | 25 | |
| Completeness [%] | 90.0 | 86.4 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 0.1M CAcodylate pH 5.8, 18% PEG4000, 0.2M Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | reservoir | cacodylate | 100 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 18 (%) |
