1G7P

CRYSTAL STRUCTURE OF MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND YEAST ALPHA-GLUCOSIDASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1999-06-16
Detector	MARRESEARCH
Wavelength(s)	1.025
Spacegroup name	P 21 21 2
Unit cell lengths	136.090, 88.344, 45.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.380 - 1.500
R-factor	0.205
Rwork	0.205
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.035	0.467
Number of reflections	82816 *	3649 *
<I/σ(I)>	33.7
Completeness [%]	92.9 *	82.3 *
Redundancy	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.25	22.5 *	sodium/potassium phosphate, MPD, pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium potassium phosphate	1.8 (M)
2	1	1	MPD	2 (%(w/v))	pH7.25