1G7I

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME (HEL) COMPLEXED WITH THE MUTANT ANTI-HEL MONOCLONAL ANTIBODY D1.3 (VLW92F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	128.270, 59.150, 56.080
Unit cell angles	90.00, 119.28, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.1828 *
Rwork	0.188
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.631
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	30405
Completeness [%]	98.3	82.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15-18 (%(w/v))
2	1	reservoir	potassium phosphate	0.1 (M)