1G71

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	129.883, 136.326, 61.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.300
R-factor	0.2108 *
Rwork	0.211
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	22.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.310	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.059	0.316
Number of reflections	46200
<I/σ(I)>	15.1	4.6
Completeness [%]	93.0	74.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5 *	20 *	0.1M Tris/Cl, 2M Ammoniumsulfate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-Cl	100 (mM)	pH8.5
2	1	reservoir	ammonium sulfate	2 (M)