1G6V

Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	83.860, 83.860, 224.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.500 - 3.500
R-factor	0.21
Rwork	0.210
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f2x 1ca2
RMSD bond length	0.008
RMSD bond angle	1.359
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.500	3.960
High resolution limit [Å]	3.500	3.500
Rmerge	0.159	0.345
Number of reflections	10533
<I/σ(I)>	4.4	2.1
Completeness [%]	99.0	99
Redundancy	5.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 8000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cAb-CA05	1.7 (mg/ml)
2	1	drop	complex	3.5 (mg/ml)
3	1	reservoir	PEG8000	25 (%(w/v))
4	1	reservoir	sodium citrate	0.1 (M)