1G6V
Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.860, 83.860, 224.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.500 | 3.960 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.159 | 0.345 |
| Number of reflections | 10533 | |
| <I/σ(I)> | 4.4 | 2.1 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 8000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cAb-CA05 | 1.7 (mg/ml) | |
| 2 | 1 | drop | complex | 3.5 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
