1G6Q

CRYSTAL STRUCTURE OF YEAST ARGININE METHYLTRANSFERASE, HMT1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	113
Detector technology	CCD
Collection date	2000-03-02
Detector	BRANDEIS
Wavelength(s)	0.9784,1.0100,0.9500,0.9788
Spacegroup name	P 1 21 1
Unit cell lengths	84.100, 129.430, 101.430
Unit cell angles	90.00, 102.74, 90.00

Refinement procedure

Resolution	28.000 - 2.900
R-factor	0.253
Rwork	0.253
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	25.060 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.990
High resolution limit [Å]	2.900	2.890
Rmerge	0.062	0.235
Total number of observations	277416 *
Number of reflections	90403
<I/σ(I)>	17.4	5
Completeness [%]	99.1	95.9
Redundancy	3.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	2 microliters of Hmt1 (8mg/ml) in 50 mM Tris, pH 7.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT + 2 microliters of reservoir:50 mM sodium hepes, pH 7.5, 14% v/v PEG 400, 100 mM CaCl2 + microseeds in 1 microliter of 20 mM sodium citrate, pH 5.6, 15% w/v PEG 4000, 100 mM ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-8 (mg/ml)
2	1	reservoir	sodium HEPES	50 (mM)
3	1	reservoir	PEG400	14 (%(v/v))
4	1	reservoir		100 (mM)
5	1	reservoir	sodium citrate	20 (mM)
6	1	reservoir	PEG4000	15 (%(w/v))
7	1	reservoir	ammonium acetate + Hmt1 microcrystals	100 (mM)