Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 253 |
| Detector technology | AREA DETECTOR |
| Collection date | 1990-03-01 |
| Detector | UCSD MARK III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.800, 95.600, 105.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GAP |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | UCSD-system |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.120 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.052 | |
| Total number of observations | 90177 * | |
| Number of reflections | 31208 | 4506 * |
| <I/σ(I)> | 14.3 | 1.37 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 298 | 0.1mM cAMP, 0.2M sodium chloride, 5mM Tris, O.1mM EDTA, 2mM DTT, .02% sodium azide, pH 7.5, MICRODIALYSIS, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8 (mg/ml) | |
| 10 | 1 | 2 | dithiothreitol | 2 (mM) | |
| 11 | 1 | 2 | sodium azide | 0.02 (%(w/v)) | |
| 12 | 1 | 1 | dithiothreitol | 2 (mM) | |
| 13 | 1 | 2 | ethylene glycol | 0-30 (%) | |
| 2 | 1 | 1 | Tris-HCl | 5 (mM) | |
| 3 | 1 | 1 | 0.2 (M) | ||
| 4 | 1 | 1 | EDTA | 0.1 (mM) | |
| 5 | 1 | 1 | sodium azide | 0.02 (%(w/v)) | |
| 6 | 1 | 2 | cAMP | 0.1 (mM) | |
| 7 | 1 | 2 | Tris-HCl | 5 (mM) | |
| 8 | 1 | 2 | 50 (mM) | ||
| 9 | 1 | 2 | EDTA | 0.1 (mM) |
