1G6G
X-RAY STRUCTURE OF THE N-TERMINAL FHA DOMAIN FROM S. CEREVISIAE RAD53P IN COMPLEX WITH A PHOSPHOTHREONINE PEPTIDE AT 1.6 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.87 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.191, 79.609, 131.467 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.377 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.610 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.068 | 0.420 |
Number of reflections | 49229 | |
<I/σ(I)> | 18.3 | |
Completeness [%] | 98.9 | 92.2 |
Redundancy | 3.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 18 * | also batch method * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mM) | |
2 | 1 | drop | sodium phosphate | 10 (mM) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | 150 (mM) | ||
5 | 1 | reservoir | mPEG2000 | 30 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
7 | 1 | reservoir | sodium acetate | 0.1 (M) |