1G6B
CRYSTAL STRUCTURE OF P47S MUTANT OF FERREDOXIN I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.970, 54.970, 92.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fd1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | 0.392 |
| Total number of observations | 83321 * | |
| Number of reflections | 11740 | |
| <I/σ(I)> | 21.8 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 * | 2 * | Shen, B., (1994) J. Biol. Chem., 269, 8564. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.5 (M) | |
| 3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.5 (M) |
