1G5T
THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. APO-ATP FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-01 |
| Detector | APS-1 |
| Wavelength(s) | 0.7009 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 49.500, 49.500, 249.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 16.800 * |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.310 |
| Number of reflections | 17014 | 1357 * |
| <I/σ(I)> | 31.6 | |
| Completeness [%] | 93.3 | 63 |
| Redundancy | 8.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.5 | 4 * | 13% me-peg 350, 100 mM NaCl, 50 mM MES, pH 5.5, batch, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | methyl ether-polyethylene glycol 350 | 13 (%) | |
| 2 | 1 | 1 | 100 (mM) | ||
| 3 | 1 | 1 | MES | 50 (mM ) | |
| 4 | 1 | 1 | protein | 5 (mg/ml) |
