1G5H
CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-10 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.623, 133.422, 135.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.714 |
| Data reduction software | MARMAD |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.072 | 0.500 |
| Number of reflections | 127238 | |
| <I/σ(I)> | 29.6 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | used microseeding * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | HEPES | 20 (mM) | |
| 4 | 1 | reservoir | sodium citrate | 0.5 (M) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) |
