1G5G

FRAGMENT OF FUSION PROTEIN FROM NEWCASTLE DISEASE VIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	1999
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	134.390, 308.330, 243.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.300
R-factor	0.224 *
Rwork	0.224
R-free	0.27300
Structure solution method	SIR + phase extension
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.220	0.880 *
Total number of observations	438164 *
Number of reflections	73130
<I/σ(I)>	7.04
Completeness [%]	96.2	87.2
Redundancy	6.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	293	unpublished data *
1	unknown *	6.5	293	unpublished data *
1	unknown *	6.5	293	unpublished data *
1	unknown *	6.5	293	unpublished data *