1G5B
BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99188, 1.0098, 1.0247 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 160.400, 177.300, 79.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.150 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.053 | 0.316 |
| Total number of observations | 435477 * | |
| Number of reflections | 61405 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 99.5 | 99.6 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100 mM MES, 20% PEG 4000, 350 mM Ammonium Sulfate, 10 mM Manganese(II) Chloride, 50 mM Ammonium Acetate, 20 mM Dithiothreitol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 23K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19-30 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | ammonium sulfate | 350 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 20 (mM) |
