1G5A
AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 1999-09-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8837, 0.9791, 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.765, 117.110, 60.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.350 - 1.400 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.174 | 0.381 |
Total number of observations | 741187 * | |
Number of reflections | 127114 | |
Completeness [%] | 93.9 | 92.3 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | Skov, L.K., (2000) Acta Crystallogr., D56, 203. * |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | Skov, L.K., (2000) Acta Crystallogr., D56, 203. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | alpha-dithiothreitol | 1 (mM) | |
6 | 1 | reservoir | PEG6000 | 30 (%) | |
7 | 1 | reservoir | HEPES | 0.1 (M) |