1G4Y
1.60 A CRYSTAL STRUCTURE OF THE GATING DOMAIN FROM SMALL CONDUCTANCE POTASSIUM CHANNEL COMPLEXED WITH CALCIUM-CALMODULIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-12-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.650, 66.690, 64.690 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.212 |
| Rwork | 0.221 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| Starting model (for MR) | room temp model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.570 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.320 |
| Total number of observations | 115320 * | |
| Number of reflections | 40694 | |
| <I/σ(I)> | 8.2 | 2.5 |
| Completeness [%] | 94.1 | 87.9 |
| Redundancy | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | ammonium sulphate, lithium sulphate, citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | 1.25 (M) | ||
| 5 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 6 | 1 | reservoir | citrate | 0.1 (M) |
