1G4R

CRYSTAL STRUCTURE OF BOVINE BETA-ARRESTIN 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-24
Detector	BRANDEIS - B4
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	77.806, 77.806, 157.099
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.940 - 2.200
R-factor	0.238
Rwork	0.238
R-free	0.26700 *
Structure solution method	MOLECULAR REPLACEMENT, SHARP
Starting model (for MR)	1cf1
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.046	0.456 *
Total number of observations	267488 *
Number of reflections	27920 *
<I/σ(I)>	10.3	1.4
Completeness [%]	94.7 *	79.2 *
Redundancy	9.6 *	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8.5 *	19 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)	or 7mg/ml
2	1	1		0.1 (M)
3	1	1	Tris-HCl	20 (mM)
4	1	1	EDTA	1 (mM)
5	1	1	dithiothreitol	10 (mM)
6	1	1	glycerol	5 (%)
7	1	2	PEG1500	18-20 (%)
8	1	2		0.8 (M)
9	1	2	Tris-HCl	0.1 (M)