1G4Q
RNA/DNA HYBRID DECAMER OF CAAAGAAAAG/CTTTTCTTTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.699, 41.067, 46.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.590 - 1.150 |
| Rwork | 0.143 |
| R-free | 0.18600 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.590 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.081 | 0.081 |
| Number of reflections | 19181 | |
| Completeness [%] | 93.5 | 65.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 4 * | Han, G.W., (1997) J.Mol.Biol., 269, 811. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | calcium acetate | ||
| 2 | 1 | 1 | spermidine | ||
| 3 | 1 | 1 | beta-octaglucoside | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA decamer | 0.17 (mM) | double helix |
| 2 | 1 | drop | magnesium acetate | 1.53 (mM) | |
| 3 | 1 | drop | spermidine | 0.17 (mM) | |
| 4 | 1 | drop | beta-octaglucoside | 0.05 (%(v/v)) | |
| 5 | 1 | drop | MPD | 9.5 (%(v/v)) |
