1G2R
Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-15 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.050, 48.750, 73.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 1.350 |
R-factor | 0.1564 |
Rwork | 0.157 |
R-free | 0.18460 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 0.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.063 | 0.269 |
Total number of observations | 101345 * | |
Number of reflections | 21402 * | |
<I/σ(I)> | 11.2 | 2.7 |
Completeness [%] | 93.5 | 61.3 |
Redundancy | 4.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 283 | 0.2 M potassium sodium tartrate, 0.1 M tri-sodium citrate, 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | potassium sodium tartrate | 0.2 (M) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2 (M) |