1G2D

STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (CLONE #2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	2000-04-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 64
Unit cell lengths	105.100, 105.100, 104.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.225 *
Rwork	0.225
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TATA structure of clone #6 solved by MIRAS
RMSD bond length	0.006
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.066 *	0.448 *
Total number of observations	358651 *
Number of reflections	32875 *
<I/σ(I)>	30.04	9.35
Completeness [%]	99.5	95.5
Redundancy	10.91	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 400, NaCl, Bis-Tris Propane, CoCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	NaCl
3	1	1	Bis-Tris Propane
4	1	1	CoCl2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	DNA	1.1 (mM)
3	1	drop	Bis-Tris-propane	100 (mM)
4	1	reservoir	PEG400	30-35 (%)
5	1	reservoir		100-200 (mM)
6	1	reservoir	Bis-Tris propane	10-50 (mM)