Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 169.500, 169.500, 113.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.266 * |
| Rwork | 0.266 |
| R-free | 0.29800 |
| Structure solution method | MIR |
| RMSD bond length | 0.023 |
| RMSD bond angle | 19.450 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.051 | 0.410 |
| Total number of observations | 264392 * | |
| Number of reflections | 57348 | |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 293 | 1.8 M (NH4)2SO4, 20 mM MgCl2, 50 mM Potassium Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | Potassium Cacodylate | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | potassium cacodylate | 50 (mM) |
