1G1W

T4 LYSOZYME MUTANT C54T/C97A/Q105M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-06-17
Detector	UCSD MARK III
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	61.093, 61.093, 96.857
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
Starting model (for MR)	1l63
RMSD bond length	0.019
RMSD bond angle	2.642
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.940
High resolution limit [Å]	1.800	1.800
Rmerge	0.072	0.152
Number of reflections	17651
<I/σ(I)>	15	3
Completeness [%]	88.5	78.6
Redundancy	2.01	1.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.1	277	Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol