1G1R
Crystal structure of P-selectin lectin/EGF domains complexed with SLeX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.140, 60.520, 91.440 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 14.000 - 3.400 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.072 | 0.322 |
| Total number of observations | 44334 * | |
| Number of reflections | 11667 | |
| <I/σ(I)> | 29 | |
| Completeness [%] | 97.8 | 100 |
| Redundancy | 3.79 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 18 * | Tris-HCl, NaCl, CaCl2, 2-methyl-2,4-pentanediol, PEG 6000, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | 12 (mM) | ||
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | drop | PEG6000 | 10 (%(w/v)) |
