1G1Q
Crystal structure of P-selectin lectin/EGF domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.000, 60.830, 91.440 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.052 | 0.225 |
| Total number of observations | 240361 * | |
| Number of reflections | 32570 | |
| <I/σ(I)> | 35.7 | |
| Completeness [%] | 95.8 | 74.2 |
| Redundancy | 7.3798 | 7.3798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 18 * | Tris-HCl, NaCl, CaCl2, 2-methyl-2,4-pentanediol, PEG 6000, pH 8.5, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | 12 (mM) | ||
| 5 | 1 | drop | MPD | 10 (%(v/v)) | |
| 6 | 1 | drop | PEG6000 | 10 (%(w/v)) |
