1G19
STRUCTURE OF RECA PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.072, 108.072, 72.783 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.218 * |
| Rwork | 0.217 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ESCHERICHIA COLI RECA (2REB) |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 2.290 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.107 | 0.537 |
| Number of reflections | 9654 | |
| <I/σ(I)> | 17.2 | 4.9 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 6.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Phosphate-Citrate, PEG 4000, AlF4, ADP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | phosphate-citrate | 100 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | PEG4000 | 6 (%) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%) | |
| 6 | 1 | reservoir | phosphate-citrate | 100 (mM) |
