1G13

HUMAN GM2 ACTIVATOR STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	150
Detector technology	CCD
Collection date	1998-05-28
Detector	ccd
Spacegroup name	P 21 21 21
Unit cell lengths	63.420, 85.630, 120.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
Rwork	0.215
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	26.130 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.049	0.330
Total number of observations	84502 *
Number of reflections	44581
<I/σ(I)>	45.7
Completeness [%]	99.8	99
Redundancy	6	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 4000, propanol, sodium Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium HEPES	0.1 (M)
2	1	reservoir	PEG4000	20 (%(v/v))
3	1	reservoir	propanol	10 (%(v/v))
4	1	drop	protein	14 (mg/ml)