1G13
HUMAN GM2 ACTIVATOR STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 1998-05-28 |
| Detector | ccd |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.420, 85.630, 120.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.130 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.330 |
| Total number of observations | 84502 * | |
| Number of reflections | 44581 | |
| <I/σ(I)> | 45.7 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 6 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, propanol, sodium Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium HEPES | 0.1 (M) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%(v/v)) | |
| 3 | 1 | reservoir | propanol | 10 (%(v/v)) | |
| 4 | 1 | drop | protein | 14 (mg/ml) |
