1G0Z
SPECIFIC MUTATIONS IN KRAIT PLA2 LEAD TO DIMERIZATION OF PROTEIN MOLECULES: CRYSTAL STRUCTURE OF KRAIT PLA2 AT 2.1 RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 180 |
Detector technology | IMAGE PLATE |
Collection date | 2000-08-22 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 80.360, 80.360, 99.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.260 - 2.180 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1FE5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.300 | 2.260 |
High resolution limit [Å] | 2.100 | 2.180 |
Number of reflections | 12475 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 7.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 281 | 50 mM Tris. HCl, 2.8 M NaCl, 1mM NaN3., pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 281K |