1G0G

CRYSTAL STRUCTURE OF T4 LYSOZYME MUTANT T152A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	1995-06-29
Detector	OTHER
Spacegroup name	P 32 2 1
Unit cell lengths	60.820, 60.820, 96.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
Rwork	0.154
RMSD bond length	0.016
RMSD bond angle	2.000
Data reduction software	SDMS
Data scaling software	UCSD-system
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.840
Rmerge	0.056	0.140
Number of reflections	16167
<I/σ(I)>	12
Completeness [%]	93.0	89
Redundancy	2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	277	Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol