1G09
CARBONMONOXY LIGANDED BOVINE HEMOGLOBIN PH 7.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-11-01 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.800, 160.200, 55.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.040 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.022 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MERLOT |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.070 | 2.190 |
| High resolution limit [Å] | 2.040 | 2.030 |
| Rmerge | 0.071 | 0.191 |
| Total number of observations | 210904 * | |
| Number of reflections | 36593 | |
| <I/σ(I)> | 10.25 | |
| Completeness [%] | 95.8 | 81 |
| Redundancy | 5.8 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.2 | 298 | sodium/potassium phosphate, pH 7.2, Batch, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | carbonmonoxyhemoglobin | 2 (%) | |
| 2 | 1 | 1 | sodium/potassium phosphate | 2.0-2.5 (M) |
