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CRYSTAL STRUCTURE OF SACCHAROMYCES CEREVISIAE UBIQUITIN CONJUGATING ENZYME 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-01 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.166, 47.471, 75.776 |
| Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 0.345 |
| Total number of observations | 85242 * | |
| Number of reflections | 23300 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 97.8 | 81.1 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 20-22 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 400 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | PEG5000 MME | 27 (%) | |
| 7 | 1 | reservoir | isopropanol | 9 (%) | |
| 8 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 9 | 1 | reservoir | MES | 100 (mM) |
