1FZR
CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.660, 135.190, 61.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.810 - 2.100 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.23500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.488 |
| Total number of observations | 229942 * | |
| Number of reflections | 61020 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | PEG 4000, ammonium sulphate, sodium chloride, Tris.HCL, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 17-19 (%(w/v)) | |
| 5 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
