1FZQ

CRYSTAL STRUCTURE OF MURINE ARL3-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	74.421, 64.583, 40.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 1.700
R-factor	0.215
Rwork	0.213
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human ARF1-GDP PDB ENTRY 1HUR
RMSD bond length	0.006
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.620	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.091	0.299
Number of reflections	22047
<I/σ(I)>	22.2	6.7
Completeness [%]	99.7	98.7
Redundancy	17.1	15.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	ammonium sulphate, MES, magnesium chloride, DTE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	29 (mg/ml)
2	1	reservoir	ammonium sulfate	1.60-1.96 (M)
3	1	reservoir	MES