1FZE
CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 1998-02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.000, 48.600, 166.400 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fza |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.145 |
| Total number of observations | 413251 * |
| Number of reflections | 35615 |
| Completeness [%] | 96.7 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | 60 (mM) | ||
| 5 | 1 | reservoir | PEG3350 | 12 (%) | |
| 6 | 1 | reservoir | sodium azide | 2 (mM) |
