1FYS
Ribonuclease T1 V16C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.445, 42.679, 56.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 2.090 - 2.000 |
| R-factor | 0.174 |
| Rwork | 0.169 |
| R-free | 0.20800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.429 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 | 0.349 |
| Total number of observations | 26858 * | |
| Number of reflections | 6623 * | |
| <I/σ(I)> | 9.22 | |
| Completeness [%] | 94.3 | 85.9 |
| Redundancy | 4.07 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 * | 298 | Zegers, I., (1998) Nat.Struct.Biol., 5, 280. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNase T1 | 15 (mg/ml) | |
| 2 | 1 | drop | exo-cGPS | 15 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | MPD | 55 (%(v/v)) |
