1FY5
Fusarium oxysporum trypsin at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.179, 36.779, 39.601 |
| Unit cell angles | 102.18, 104.50, 103.12 |
Refinement procedure
| Resolution | 20.000 - 0.810 |
| R-factor | 0.1242 |
| Rwork | 0.124 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.820 |
| High resolution limit [Å] | 0.810 | 0.810 |
| Rmerge | 0.082 | 0.367 |
| Number of reflections | 1208333 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 96.4 | 85 |
| Redundancy | 7.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
