1FY5
Fusarium oxysporum trypsin at atomic resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-18 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 33.179, 36.779, 39.601 |
Unit cell angles | 102.18, 104.50, 103.12 |
Refinement procedure
Resolution | 20.000 - 0.810 |
R-factor | 0.1242 |
Rwork | 0.124 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.034 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.820 |
High resolution limit [Å] | 0.810 | 0.810 |
Rmerge | 0.082 | 0.367 |
Number of reflections | 1208333 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 96.4 | 85 |
Redundancy | 7.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |